Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
Cross-Reactivity (Details)
Cross reactivity with PTRF (polymerase I and transcript release factor) from other sources has not been determined.
Purity
Anti-PTRF was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards Cavin 1 PTRF. A BLAST analysis was used to suggest cross-reactivity with Human based on 100 % sequence homology.
Endotoxin Level
Low Endotoxin : No
Immunogen
PTRF affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human PTRF. Immunogen Type: Peptide
PTRF
Reactivity: Human
ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Anti-PTRF antibody is useful for ELISA, Immunostaining, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Protein Molecular weight: 60 kDa. Expect a band approximately ~40.5 kDa corresponding to the appropriate cell lysate or extract. ELISA Dilution: 1:20.000 - 1:60.000 Immunohistochemistry Dilution: 1:100-1:500 IF Microscopy Dilution: 1:100-1:500 Western Blot Dilution: 1 µg/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
1.06 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 50 % (v/v) Glycerol
Storage
4 °C/-20 °C
Storage Comment
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date
Expiration date is six (6) months from date of opening.
Target
PTRF
(Polymerase I and Transcript Release Factor (PTRF))
PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13. Anti-PTRF antibodies are ideal for researchers interested in Diabetes Research, Lipid and Metabolism research. Synonyms: CAVIN, cavin-1, Cavin-1, CGL4, FLJ90031, polymerase I and transcript release factor, RNA polymerase I and transcript release factor, TTF-I interacting peptide 12