PTRF antibody (C-Term)

Catalog No. ABIN1607897

Product Details anti-PTRF Antibody

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with PTRF (polymerase I and transcript release factor) from other sources has not been determined.

Purity: Anti-PTRF was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards Cavin 1 PTRF. A BLAST analysis was used to suggest cross-reactivity with Human based on 100 % sequence homology.

Endotoxin Level: Low Endotoxin : No

Immunogen: PTRF affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human PTRF.
Immunogen Type: Peptide

Application Details

Application Notes: Anti-PTRF antibody is useful for ELISA, Immunostaining, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Protein Molecular weight: 60 kDa. Expect a band approximately ~40.5 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 1 µg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is six (6) months from date of opening.

Target Details for PTRF

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Alternative Name: PTRF (PTRF Products)

Synonyms: MGC53017 antibody, MGC89141 antibody, PTRF antibody, fd20g07 antibody, ptrf antibody, wu:fd20g07 antibody, zgc:162917 antibody, CAVIN antibody, CAVIN1 antibody, CGL4 antibody, cavin-1 antibody, 2310075E07Rik antibody, AW546441 antibody, Cav-p60 antibody, Cavin antibody, caveolae associated protein 1 L homeolog antibody, polymerase I and transcript release factor antibody, caveolae associated protein 1 antibody, caveolae associated protein 1b antibody, caveolae associated 1 antibody, cavin1.L antibody, PTRF antibody, cavin1 antibody, CAVIN1 antibody, cavin1b antibody, Cavin1 antibody

Background: PTRF (polymerase I and transcript release factor) was first identified as a TTF-I and RNA polymerase I (RNA Pol I) interacting protein that functions in the termination of RNA polymerase I transcription. PTRF has also been described as a protein that localizes to the plasma membrane and caveolae of adipocytes and whose localization is under the control of insulin. In this context, PTRF has been observed to associate with a lipase and have an extranuclear role in the regulation of lipolysis. PTRF is also known as FKSG13. Anti-PTRF antibodies are ideal for researchers interested in Diabetes Research, Lipid and Metabolism research. Synonyms: CAVIN, cavin-1, Cavin-1, CGL4, FLJ90031, polymerase I and transcript release factor, RNA polymerase I and transcript release factor, TTF-I interacting peptide 12

Gene ID: 284119

NCBI Accession: NP_036364

UniProt: Q6NZI2