EIF3A antibody (AA 1-250)

Catalog No. ABIN1679692

This Rabbit Polyclonal antibody specifically detects EIF3A in WB and IF. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for EIF3A antibody (AA 1-250) (ABIN1679692)

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-EIF3A Antibody

Binding Specificity: AA 1-250

Sequence: MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDVV RAYLKMAEEK TEAAKEESQQ MVLDIEDLDN IQTPESVLLS AVSGEDTQDR TDRLLLTPWV KFLWESYRQC LDLLRNNSRV ERLYHDIAQQ AFKFCLQYTR KAEFRKLCDN LRMHLSQIQR HHNQSTAINL NNPESQSMHL ETRLVQLDSA ISMELWQEAF

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF3A (NP_003741.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:1000,IF,1:50 - 1:100

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-EIF3A antibody (ABIN1679692)

Yang, Hu, Fan, Zhang, Zhong, Guo, Qin, Chen: "Picornavirus 2A protease regulates stress granule formation to facilitate viral translation." in: PLoS pathogens, Vol. 14, Issue 2, pp. e1006901, (2018) (PubMed).

Target Details for EIF3A

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Alternative Name: EIF3A

Background: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815).,EIF3A,EIF3,EIF3S10,P167,TIF32,eIF3-p170,eIF3-theta,p180,p185,Epigenetics & Nuclear Signaling,RNA Binding,Translation Control,EIF3A

Molecular Weight: 162 kDa/166 kDa

Gene ID: 8661

UniProt: Q14152

Pathways: Ribonucleoprotein Complex Subunit Organization