EIF3A antibody (C-Term)

Catalog No. ABIN2788872

This Rabbit Polyclonal antibody specifically detects EIF3A in WB. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Dog, Zebrafish (Danio rerio), Horse and Pig.

Quick Overview for EIF3A antibody (C-Term) (ABIN2788872)

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Zebrafish (Danio rerio), Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF3A Antibody

Binding Specificity: C-Term

Purpose: EIF3A Antibody - C-terminal region

Sequence: PPALSRDRER DRDREREGEK EKASWRAEKD RESLRRTKNE TDEDGWTTVR

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against EIF3A. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP61702-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3A

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Alternative Name: EIF3A

Background:

Targeted Subunit: A

Background Information: EIF3A is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Gene Name: Eukaryotic translation initiation factor 3, subunit A

Alternative Symbols: EIF3, P167, p180, p185, TIF32, EIF3S10, eIF3-p170, eIF3-theta

Protein Name: Eukaryotic translation initiation factor 3 subunit A

Molecular Weight: 152kDa

Gene ID: 8661

NCBI Accession: NP_003741

UniProt: Q14152

Pathways: Ribonucleoprotein Complex Subunit Organization