CREB1 antibody (Ser133)

Catalog No. ABIN1685160

This Mouse Polyclonal antibody specifically detects CREB1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CREB1 antibody (Ser133) (ABIN1685160)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Polyclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CREB1 Antibody

Binding Specificity: Ser133

Purification: Purified

Sterility: Sterile filtered

Immunogen: Polyclonal p-CREB-1 antibody was produced from sera of mice immunized with synthetic protein containing residues surrounding ser133 .

Isotype: IgG

Application Details

Application Notes: ELISA: Use at a concentration range of 1-2 µg/mL.
Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL. WB analysis mouse hippocampal tissue lysate expressing phospho CREB1. ABIN1685160 was used as primary antibody.
IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL. Immunohistochemical analysis of frozen mouse hyppocampal sections expressing CREB1. ABIN1685160 was used as primary antibody, dilution 1:200. Immunohistochemical analysis of FFPE mouse thymocytes expressing phospho CREB-1. ABIN1685160 was used as primary antibody, dilution 1:500.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers

Buffer: PBS solution at a concentration of 1 mg/mL.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Recombinant IL-21R, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.

Expiry Date: 12 months

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1

Background: The IL-21 receptor (IL-21R) is structurally related to IL-2R and IL-15R. In addition, these receptors, together with IL-4R, IL-7R, and IL-9R, share a common gamma -chain. IL-21R appears in T, natural killer (NK), B, and dendritic cells (DC), and it plays an important role in the regulation and function of these cells. IL-21 has, however, an effect on NK cells depending on their activation state. It enhances the proliferation and cytotoxic activity of activated NK cells, but in these cells, IL-21 also promotes apoptosis and thus limits NK cell response. In contrast, IL-21 inhibits IL-15-induced proliferation and maturation of NK cell precursors

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation