CREB1 antibody (pSer133)

Catalog No. ABIN7201902

The Rabbit Polyclonal anti-CREB1 antibody is suitable to detect CREB1 in samples from Human, Mouse, Rat and Pig. It has been validated for WB, IHC, ELISA, IF and IP.

Quick Overview for CREB1 antibody (pSer133) (ABIN7201902)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-CREB1 Antibody

Binding Specificity: pSer133

Purpose: Rabbit Anti-CREB-1 (phospho Ser133) Polyclonal Antibody

Specificity: Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CREB-1 Phospho-Ser133

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,IHC 1:100-1:300,IP 2-5 μg:mg lysate,ELISA 1:10000,Not yet tested in other applications,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB-1

Background: CREB1, Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1CREB1 encodes a transcription factor (cAMP responsive element binding protein 1) that is a member of the leucine zipper family of DNA binding proteins. cAMP responsive element binding protein 1 binds as a homodimer to the cAMP-responsive element, an octameric palindrome. cAMP responsive element binding protein 1 is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of CREB1 results in several transcript variants encoding different isoforms.

Molecular Weight: 37kD

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation