POLK antibody (AA 1-100) (AbBy Fluor® 647)

Catalog No. ABIN1699415

The Rabbit Polyclonal anti-POLK antibody has been validated for FACS. It is suitable to detect POLK in samples from Human.

Quick Overview for POLK antibody (AA 1-100) (AbBy Fluor® 647) (ABIN1699415)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLK antibody is conjugated to AbBy Fluor® 647

Application: Flow Cytometry (FACS)

Product Details anti-POLK Antibody (AbBy Fluor® 647)

Binding Specificity: AA 1-100

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA Polymerase Kappa/POLK

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: DNA Polymerase Kappa/POLK

Background:

Synonyms: DNA polymerase kappa, DINB protein, Short=DINP, POLK_HUMAN.

Background: DNA polymerase lambda (pol Kappa), also designated DNA polymerase Kappa or Pol Beta2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol Kappa is a member of the DNA polymerase type-X family. Pol Kappa extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol Kappa may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol Kappa and thus influences the ability of pol Kappa to synthesize DNA.

Gene ID: 51426

Pathways: DNA Damage Repair