FZR1 antibody (AA 141-240) (Cy3)

Catalog No. ABIN1702483

This Rabbit Polyclonal antibody specifically detects FZR1 in WB, IF (cc) and IF (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for FZR1 antibody (AA 141-240) (Cy3) (ABIN1702483)

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZR1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-FZR1 Antibody (Cy3)

Binding Specificity: AA 141-240

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FZR1/CDC20C

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FZR1

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Alternative Name: FZR1/CDC20C

Background:

Synonyms: FZR, FZR2, CDC20C, Fizzy-related protein homolog, CDC20-like protein 1, Cdh1/Hct1 homolog, FZR1, FYR, KIAA1242

Background: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.

Gene ID: 51343

UniProt: Q9UM11

Pathways: DNA Replication, Synthesis of DNA