ZBTB7A antibody (AA 321-420) (Cy3)

Catalog No. ABIN1702532

The Rabbit Polyclonal anti-ZBTB7A antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect ZBTB7A in samples from Human.

Quick Overview for ZBTB7A antibody (AA 321-420) (Cy3) (ABIN1702532)

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBTB7A antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ZBTB7A Antibody (Cy3)

Binding Specificity: AA 321-420

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Pokemon

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ZBTB7A

Target: ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))

Alternative Name: Pokemon/ZBTB7

Background:

Synonyms: Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, FBI1, HIV-1 1st-binding protein 1, Leukemia/lymphoma related factor, LRF, Pokemon, TIP21, TTF-I interacting peptide 21, ZBTB7, ZBTB7A, Zinc finger and BTB domain-containing protein 7A, ZBT7A_HUMAN.

Background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

Gene ID: 51341