HEMGN antibody (AA 51-150) (HRP)

Catalog No. ABIN1711043

The Rabbit Polyclonal anti-HEMGN antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect HEMGN in samples from Human.

Quick Overview for HEMGN antibody (AA 51-150) (HRP) (ABIN1711043)

Target: HEMGN (Hemogen (HEMGN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HEMGN antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HEMGN Antibody (HRP)

Binding Specificity: AA 51-150

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Hemogen/EDAG

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HEMGN

Target: HEMGN (Hemogen (HEMGN))

Alternative Name: Hemogen/EDAG

Background:

Synonyms: EDAG, EDAG-1, Erythroid dferentiation-associated gene protein, HEMGN, HEMGN_HUMAN, Hemogen, Hemopoietic gene protein, Negative dferentiation regulator protein.

Background: Hemogen is a 484 amino acid protein encoded by the human gene HEMGN. Hemogen is a nuclear protein that is expressed in hematopoietic precursor cells and can be detected in CD34+ and K-562 leukemia cell line. It is also expressed in bone marrow, testis, thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia. Hemogen is down-regulated during megakaryocytic differentiation of K-562 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) (at protein level). It can be up-regulated in normal PBMCs by mitogens.

Gene ID: 55363