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HEMGN antibody (AA 51-150) (HRP)

The Rabbit Polyclonal anti-HEMGN antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect HEMGN in samples from Human.
Catalog No. ABIN1711043

Quick Overview for HEMGN antibody (AA 51-150) (HRP) (ABIN1711043)

Target

See all HEMGN Antibodies
HEMGN (Hemogen (HEMGN))

Reactivity

  • 30
  • 2
  • 1
Human

Host

  • 28
  • 2
Rabbit

Clonality

  • 28
  • 2
Polyclonal

Conjugate

  • 12
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This HEMGN antibody is conjugated to HRP

Application

  • 30
  • 13
  • 13
  • 10
  • 3
  • 3
  • 3
  • 2
  • 2
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Binding Specificity

    • 14
    • 8
    • 3
    • 2
    • 1
    • 1
    • 1
    AA 51-150

    Predicted Reactivity

    Human

    Purification

    Purified by Protein A.

    Immunogen

    KLH conjugated synthetic peptide derived from human Hemogen/EDAG

    Isotype

    IgG
  • Application Notes

    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Buffer

    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handling Advice

    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

    Expiry Date

    12 months
  • Target

    HEMGN (Hemogen (HEMGN))

    Alternative Name

    Hemogen/EDAG

    Background

    Synonyms: EDAG, EDAG-1, Erythroid dferentiation-associated gene protein, HEMGN, HEMGN_HUMAN, Hemogen, Hemopoietic gene protein, Negative dferentiation regulator protein.

    Background: Hemogen is a 484 amino acid protein encoded by the human gene HEMGN. Hemogen is a nuclear protein that is expressed in hematopoietic precursor cells and can be detected in CD34+ and K-562 leukemia cell line. It is also expressed in bone marrow, testis, thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia. Hemogen is down-regulated during megakaryocytic differentiation of K-562 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) (at protein level). It can be up-regulated in normal PBMCs by mitogens.

    Gene ID

    55363
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