AFAP1L2 antibody (AA 751-818) (HRP)

Catalog No. ABIN1711211

The Rabbit Polyclonal anti-AFAP1L2 antibody has been validated for WB, ELISA. It is suitable to detect AFAP1L2 in samples from Mouse.

Quick Overview for AFAP1L2 antibody (AA 751-818) (HRP) (ABIN1711211)

Target: AFAP1L2 (Actin Filament Associated Protein 1-Like 2 (AFAP1L2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFAP1L2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-AFAP1L2 Antibody (HRP)

Binding Specificity: AA 751-818

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AFAP1L2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AFAP1L2

Target: AFAP1L2 (Actin Filament Associated Protein 1-Like 2 (AFAP1L2))

Alternative Name: AFAP1L2

Background:

Synonyms: KIAA1914, XB130, Actin filament-associated protein 1-like 2, AF1L2_HUMAN, AFAP1-like protein 2, afap1l2.

Background: Actin filament associated protein (AFAP-110) interacts directly with actin filaments through its C-terminal actin-binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. By linking signaling molecules to actin filaments, AFAP-110 provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. AFAP-1L2 (Actin filament-associated protein 1-like 2), also known as XB130, is a 818 amino acid cytoplasmic protein that contains two Pleckstrin homology (PH) domains, which are normally found in proteins involved in intracellular signaling. Like its relative AFAP110, AFAP-1L2 interacts with Src kinase and may play a role in Src-regulated transcription activation. AFAP-1L2 is expressed in thyroid and spleen and can also be detected at lower levels in lung, brain, pancreas and kidney. There are four isoforms of AFAP-1L2 that are produced as a result of alternative splicing events.

Pathways: EGFR Signaling Pathway