AFAP1L2 antibody (C-Term)

Catalog No. ABIN499229

The Rabbit Polyclonal anti-AFAP1L2 antibody is suitable to detect AFAP1L2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p) and EIA.

Quick Overview for AFAP1L2 antibody (C-Term) (ABIN499229)

Target: AFAP1L2 (Actin Filament Associated Protein 1-Like 2 (AFAP1L2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFAP1L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-AFAP1L2 Antibody

Binding Specificity: C-Term

Specificity: This anitbody reacts to AFAP1L2.

Purification: Affinity chromatography purified via peptide column

Immunogen: AFAP1L2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human AFAP1L2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: AFAP1L2 antibody can be used for detection of AFAP1L2 at 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for AFAP1L2

Target: AFAP1L2 (Actin Filament Associated Protein 1-Like 2 (AFAP1L2))

Alternative Name: AFAP1L2

Background: AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3b, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.Synonyms: AFAP1-like protein 2, Actin filament-associated protein 1-like 2, KIAA1914, XB130

Gene ID: 84632

NCBI Accession: NP_001001936

UniProt: Q8N4X5

Pathways: EGFR Signaling Pathway