IKAP/p150 antibody (AA 1151-1250) (HRP)

Catalog No. ABIN1711362

This anti-IKAP/p150 antibody is a Rabbit Polyclonal antibody detecting IKAP/p150 in WB, ELISA, IHC (p) and IHC (fro). Suitable for Mouse and Rat.

Quick Overview for IKAP/p150 antibody (AA 1151-1250) (HRP) (ABIN1711362)

Target: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKAP/p150 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IKAP/p150 Antibody (HRP)

Binding Specificity: AA 1151-1250

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IKAP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for IKAP/p150

Target: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Alternative Name: IKAP

Background:

Synonyms: DKFZp781H1425, DYS, Dysautonomia Riley Day syndrome hereditary sensory autonomic neuropathy type III, Elongator complex protein 1, ELP 1, ELP1, ELP1_HUMAN, FD, FLJ12497, IKAP, IkappaB kinase complex associated protein, IkappaB kinase complex-associated protein, ikbkap, IKI 3, IKI3, IKK complex associated protein, IKK complex-associated protein, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein, OTTHUMP00000063889, p150, TOT 1, TOT1

Background: The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in the targeted degradation of IkB (1). IkB kinase alpha (IKK alpha), previously designated CHUK (2), interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation, serines 32 and 36 (3). IKKalpha appears to be critical for NFkB activation in response to proinflammatory cytokines (4). Phosphorylation of the IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1 (5). The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma (also designated NEMO), and each appears to make essential contributions to IkB phosphorylation (6). IKAP (IKK-complex-associated protein) is a protein that acts as a scaffold, interacting with NIK, IKK alpha and IKK beta and assembling them into an active kinase complex (7,8)