MAP4K5 antibody (AA 751-846) (HRP)

Catalog No. ABIN1711376

The Rabbit Polyclonal anti-MAP4K5 antibody is suitable to detect MAP4K5 in samples from Mouse. It has been validated for WB, ELISA, IHC (p) and IHC (fro).

Quick Overview for MAP4K5 antibody (AA 751-846) (HRP) (ABIN1711376)

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K5 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAP4K5 Antibody (HRP)

Binding Specificity: AA 751-846

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MAP4K5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MAP4K5

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Alternative Name: MAP4K5

Background:

Synonyms: EC 2.7.11.1, KHS, Kinase homologous to SPS1/STE20, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5, MEKKK 5, M4K5_HUMAN.

Background: Several mammalian kinases have been identified with sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include KHS, GLK, NIK, YSK1, HPK1, Krs-1, Krs-2 and GC kinase. KHS (for kinase homologous to SPS1/STE20) is a protein that is most closely related to GC kinase. The KHS kinase has been shown to activate a variety of substrates, including JNK, suggesting a role in stress response.