APOO antibody (AA 101-198) (HRP)

Catalog No. ABIN1711622

The Rabbit Polyclonal anti-APOO antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect APOO in samples from Mouse and Rat.

Quick Overview for APOO antibody (AA 101-198) (HRP) (ABIN1711622)

Target: APOO (Apolipoprotein O (APOO))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOO antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APOO Antibody (HRP)

Binding Specificity: AA 101-198

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ApoO/Apolipoprotein O

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for APOO

Target: APOO (Apolipoprotein O (APOO))

Alternative Name: Apolipoprotein O

Background:

Synonyms: ApoO, 0610008C08Rik, 1110019O03Rik, Apolipoprotein O, APOO, APOO_HUMAN, FAM121B, Family with sequence similarity 121B, MGC130105, MGC130106, MGC4825, My025, MYO25, OTTHUMP00000023073, Protein FAM121B, RP23-272D10.2.

Background: Apolipoproteins are a family of fatty-acid binding proteins that transport fat through the bloodstream in the form of lipoproteins. ApoO (Apolipoprotein O), also known as FAM121B or My025, is a 198 amino acid single-pass membrane protein that belongs to the apolipoprotein family. Expressed ubiquitously with particularly high expression in diabetic heart tissue, apoO functions to promote the transport of cholesterol from macrophage cells and may be involved in regulatory mechanisms that protect lipid accumulation within the heart. ApoO is present in high density lipoproteins (HDLs) and low density lipoproteins (LDLs) and is secreted by an MTP (microsomal triglyceride transfer protein)-dependent mechanism. Two isoforms of apoO exist due to alternative splicing events.

Gene ID: 79135