POLL antibody (AA 475-575) (HRP)

Catalog No. ABIN1711659

This anti-POLL antibody is a Rabbit Polyclonal antibody detecting POLL in WB, ELISA, IHC (p) and IHC (fro). Suitable for Rat.

Quick Overview for POLL antibody (AA 475-575) (HRP) (ABIN1711659)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLL antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-POLL Antibody (HRP)

Binding Specificity: AA 475-575

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA Polymerase lambda

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: DNA Polymerase lambda

Background:

Synonyms: BETA N, BETAN, DNA directed DNA polymerase lambda, DNA polymerase beta 2, DNA polymerase beta-2, DNA polymerase kappa, DNA polymerase kappa DNA polymerase beta N, DNA polymerase lambda, DNA polymerase lamda2, DPOLL_HUMAN, EC 2.7.7.7,EC 4.2.99., FLJ46002, OTTHUMP00000020321, OTTHUMP00000020323, OTTHUMP00000059179, Pol beta2, POL KAPPA, Pol Lambda, POLKAPPA, POLL, Polymerase DNA directed lambda.

Background: DNA polymerase lambda (pol lambda), also designated DNA polymerase lambda or Pol Beta2, is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. Encoded by the POLL gene, pol lambda is a member of the DNA polymerase type-X family. Pol lambda extends primer-terminal mispairs opposite nondamaged DNA templates, suggesting that it may assist in extending mismatched base pairs during normal DNA replication. In addition, pol ?may play a role in the mutagenic bypass of T-T dimers. Proliferating cell nuclear antigen (PCNA), a protein essential to DNA replication, interacts with pol lambda and thus influences the ability of pol ?to synthesize DNA.

Gene ID: 27343

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response