POLL antibody (C-Term)

Catalog No. ABIN185143

Product Details anti-POLL Antibody

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This POLL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: POLL / POLkappa

Sequence: LGLPYREPAE RDW

Specificity: It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-LGLPYREPAERDW, from the C Terminus of the protein sequence according to NP_037406.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in cell lysates of A549 and approx 60-70 kDa in cell lysates of HepG2 (calculated MW of 63.5 kDa according to Human NP_037406.1). Recommended concentration: 0.5-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: POLL (POLL Products)

Synonyms: T10O24.13 antibody, T10O24_13 antibody, zgc:66320 antibody, zgc:77901 antibody, wu:fe49a04 antibody, beta-n antibody, pol-kappa antibody, BETAN antibody, POLKAPPA antibody, 1110003P06Rik antibody, AV007317 antibody, DNA polymerase lambda (POLL) antibody, DNA polymerase lambda antibody, polymerase (DNA directed), lambda antibody, polymerase (DNA) lambda antibody, Pol{lambda} antibody, PTRG_00468 antibody, poll antibody, POLL antibody, MCYG_03796 antibody, LOC9325878 antibody, MGYG_02138 antibody, Poll antibody

Background: POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA

Gene ID: 27343, 56626

NCBI Accession: NP_037406

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response