FAN1 antibody (HRP)

Catalog No. ABIN1711673

The Rabbit Polyclonal anti-FAN1 antibody has been validated for WB and IHC (p). It is suitable to detect FAN1 in samples from Human, Mouse and Rat.

Quick Overview for FAN1 antibody (HRP) (ABIN1711673)

Target: FAN1 (FANCD2/FANCI-Associated Nuclease 1 (FAN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAN1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FAN1 Antibody (HRP)

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FAN1

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % Gentamicin.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Storage Comment: Store at 4°C

Expiry Date: 12 months

Target Details for FAN1

Target: FAN1 (FANCD2/FANCI-Associated Nuclease 1 (FAN1))

Alternative Name: Fan1

Background:

Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771). Also has endonuclease activity toward 5'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866).

Subcellular location: Nucleus

Synonyms: KMIN, hFAN1, MTMR15, KIAA1018, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15, FAN1

Gene ID: 22909

UniProt: Q9Y2M0

Pathways: DNA Damage Repair