APOBEC3C antibody (AA 121-190)

Catalog No. ABIN1713363

This anti-APOBEC3C antibody is a Rabbit Polyclonal antibody detecting APOBEC3C in WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) and ICC. Suitable for Human.

Quick Overview for APOBEC3C antibody (AA 121-190) (ABIN1713363)

Target: APOBEC3C (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3C (APOBEC3C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOBEC3C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-APOBEC3C Antibody

Binding Specificity: AA 121-190

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APOBEC3C

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for APOBEC3C

Target: APOBEC3C (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3C (APOBEC3C))

Alternative Name: APOBEC3C

Background:

Synonyms: ABC3C_HUMAN, APOBEC1 like, APOBEC1-like, APOBEC1L, APOBEC3C, Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3C, ARDC2, ARDC4, ARP5, bK150C2.3, MGC19485, PBI, Phorbolin I, Phorbolin I protein, Probable DNA dC dU editing enzyme APOBEC 3C, Probable DNA dC->dU-editing enzyme APOBEC-3C.

Background: APOBEC proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA. The seven APOBEC3 genes or pseudogenes are found in a cluster thought to result from gene duplication on chromosome 22. Like APOBEC3G, APOBEC3F deaminates deoxycytosine to deoxyuracil in the minus strand of HIV-1 DNA, resulting in G to A hypermutation in the plus strand of DNA. Thus, APOBEC3G and APOBEC3F provide a mechanism for innate immunity to retroviruses, and are also likely contribute to sequence variation observed in many viruses. Viral infectivity factor (Vif) imparts APOBEC3G and APOBEC3F resistance to HIV through impaired translation of their mRNA and accelerated posttranslational degradation of the APOBEC3 proteins by the 26S proteasome. Interestingly, HIV-1 Vif cannot form a complex with APOBEC3G or APOBEC3F of mouse origin as it does with the human protein, and thus mouse APOBEC3G and APOBEC3F function as a potent inhibitors of wildtype HIV-1 replication, where human APOBEC3G and APOBEC3F are only able to inhibit Vif-deficient HIV-1 replication. This implies that induction of APOBEC3G and APOBEC3F activity or a method of blocking their interaction with Vif may provide a method for therapeutic intervention.

Gene ID: 27350