Hect E3 Ubiquitin Ligase antibody (AA 1-100)

Catalog No. ABIN1713527

The Rabbit Polyclonal anti-Hect E3 Ubiquitin Ligase antibody has been validated for ELISA, ICC, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect Hect E3 Ubiquitin Ligase in samples from Rat.

Quick Overview for Hect E3 Ubiquitin Ligase antibody (AA 1-100) (ABIN1713527)

Target: Hect E3 Ubiquitin Ligase (LOC662402)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Hect E3 Ubiquitin Ligase Antibody

Binding Specificity: AA 1-100

Cross-Reactivity: Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HECT E3 ubiquitin ligase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Hect E3 Ubiquitin Ligase

Target: Hect E3 Ubiquitin Ligase (LOC662402)

Alternative Name: HECT E3 ubiquitin ligase

Background:

Synonyms: CEB1, CEBP1, Cyclin E binding protein 1, Cyclin-E-binding protein 1, E3 ISG15--protein ligase HERC5, HECT domain and RCC1 like domain containing protein 5, HECT domain and RCC1-like domain-containing protein 5, Hect domain and RLD 5, HECT E3 ubiquitin ligase, HERC5, HERC5_HUMAN, Herc6.

Background: HECT E3 ubiquitin ligase is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.

Gene ID: 51191