GCNT2 antibody (AA 151-250)

Catalog No. ABIN1713810

The Rabbit Polyclonal anti-GCNT2 antibody (ABIN1713810) specifically detects GCNT2 in WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for GCNT2 antibody (AA 151-250) (ABIN1713810)

Target: GCNT2 (Glucosaminyl (N-Acetyl) Transferase 2, I-Branching Enzyme (I Blood Group) (GCNT2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCNT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-GCNT2 Antibody

Binding Specificity: AA 151-250

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GCNT2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GCNT2

Target: GCNT2 (Glucosaminyl (N-Acetyl) Transferase 2, I-Branching Enzyme (I Blood Group) (GCNT2))

Alternative Name: GCNT2

Background:

Synonyms: bA360O19.2, bA421M1.1, Beta 1 6 N acetylglucosaminyltransferase 2, CCAT, GCNT 2, GCNT2C, GCNT5, Glucosaminyl N acetyl transferase 2 I branching enzyme I blood group, Glucosaminyl N acetyl transferase 2 I branching enzyme, I beta 1 6 N acetylglucosaminyltransferase, I branching beta 1 6 acetylglucosaminyltransferase, I branching enzyme, IGNT, II, Ii blood group, MGC163396, N acetylglucosaminyltransferase, N acetyllactosaminide beta 1 6 N acetylglucosaminyltransferase, NACGT1, NAGCT1, ULG3.

Background: Belonging to the glycosyltransferase 14 family, GCNT2 (glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)), also known as II, N-acetylglucosaminyltransferase, IGNT, CCAT, ULG3, GCNT5, GCNT2C or NACGT1, is a 400 amino acid glycosyltransferase that localizes to the Golgi apparatus. Other members of the glycosyltransferase 14 family include GCNT1, GCNT3, GCNT4, GCNT6 and GCNT7. A single-pass type II membrane protein, GCNT2 functions as a branching enzyme known as beta-1,6-N-acetylglucosaminyltransferase, which converts fetal i antigen to adult I antigen in erythrocytes during embryonic development. With expression levels increasing significantly during oncogenesis and development, GCNT2 is found at highest levels in adult prostate and fetal brain, and is found at low levels in heart, small intestine, colon, brain, pancreas and kidney.

Gene ID: 2651

Pathways: Glycosaminoglycan Metabolic Process