GRB14 antibody (AA 221-320)

Catalog No. ABIN1713832

This Rabbit Polyclonal antibody specifically detects GRB14 in ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for GRB14 antibody (AA 221-320) (ABIN1713832)

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB14 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GRB14 Antibody

Binding Specificity: AA 221-320

Specificity: This antibody may also detect the similar protein GRB10 due to an 83 % sequence similarity in the immunogen sequence.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GRB 14

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GRB14

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Alternative Name: GRB 14

Background:

Synonyms: Growth factor receptor-bound protein 14, GRB14 adapter protein, GRB14

Background: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.

Gene ID: 2888

UniProt: Q14449