GRB14 antibody (Internal Region)

Catalog No. ABIN6258228

The Rabbit Polyclonal anti-GRB14 antibody is suitable to detect GRB14 in samples from Human, Mouse and Rat. It has been validated for WB, IF and ICC.

Quick Overview for GRB14 antibody (Internal Region) (ABIN6258228)

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GRB14 Antibody

Binding Specificity: Internal Region

Purpose: GRB14 Antibody

Specificity: GRB14 Antibody detects endogenous levels of total GRB14.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GRB14, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GRB14

Target: GRB14 (Growth Factor Receptor-Bound Protein 14 (GRB14))

Alternative Name: GRB14

Background:

Description: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.

Gene: GRB14

Molecular Weight: 61 kDa

Gene ID: 2888

UniProt: Q14449