MHC Class II antibody (AA 111-200)

Catalog No. ABIN1713842

The Rabbit Polyclonal anti-MHC Class II antibody is suitable to detect MHC Class II in samples from Mouse and Human. It has been validated for WB, IHC (fro), ICC, IHC (p), IF (cc), IF (p) and ELISA.

Quick Overview for MHC Class II antibody (AA 111-200) (ABIN1713842)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MHC Class II antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA

Product Details anti-MHC Class II Antibody

Binding Specificity: AA 111-200

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC Class II

Background:

Synonyms: RING7, D6S221E, HLA class II histocompatibility antigen, DM beta chain, MHC class II antigen DMB, Really interesting new gene 7 protein, HLA-DMB, DMB

Background: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.

Gene ID: 3109

UniProt: P28068