ERF antibody (AA 65-160)

Catalog No. ABIN1714151

Product Details anti-ERF Antibody

Target: ERF (Ets2 Repressor Factor (ERF))

Binding Specificity: AA 65-160

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ERF/Ets2 Repressor Factor

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ERF

Target: ERF (Ets2 Repressor Factor (ERF))

Alternative Name: ERF/Ets2 Repressor Factor (ERF Products)

Synonyms: CRS4 antibody, PE-2 antibody, PE2 antibody, fc22h01 antibody, wu:fc22h01 antibody, ETF1 antibody, erf antibody, ETS2 repressor factor antibody, Ets2 repressor factor antibody, ETS2 repressor factor S homeolog antibody, ERF antibody, erf antibody, Erf antibody, erf.S antibody

Background:

Synonyms: ERF, ERF_HUMAN, ETS domain containing transcription factor ERF, ETS domain-containing transcription factor ERF, Ets2 repressor factor, PE 2, PE-2, PE2.

Background: ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters. ABT1 associates with TBP in HeLa nuclear extracts in vitro. Another protein, designated ERF, is a member of the Ets family of transcription factors. The members of the Ets family are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. ERF (Ets-2 repressor factor) is a ubiquitously expressed Ets-domain protein that exhibits strong transcriptional repressor activity, suppresses Ets-induced transformation and is regulated by MAPK phosphorylation. ERF transcription may be regulated by Ets-domain proteins. Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase.

Gene ID: 2077