FZD3 antibody (AA 121-220)

Catalog No. ABIN1714171

This Rabbit Polyclonal antibody specifically detects FZD3 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Xenopus laevisand has been mentioned in 1 publication.

Quick Overview for FZD3 antibody (AA 121-220) (ABIN1714171)

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-FZD3 Antibody

Binding Specificity: AA 121-220

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FZD3 antibody (ABIN1714171)

Su, Zhang, Gan, Jiang, Xiao, Zou, Li, Jiang: "Deregulation of the planar cell polarity genes CELSR3 and FZD3 in Hirschsprung disease." in: Experimental and molecular pathology, Vol. 101, Issue 2, pp. 241-248, (2016) (PubMed).

Target Details for FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Alternative Name: FZD3/Frizzled 3

Background:

Synonyms: Fz-3, Frizzled-3, hFz3, FZD3

Background: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Gene ID: 7976

UniProt: Q9NPG1

Pathways: WNT Signaling, Tube Formation