GABPA antibody (AA 51-150)

Catalog No. ABIN1714176

Cited in 1 publication. The Rabbit Polyclonal anti-GABPA antibody (ABIN1714176) specifically detects GABPA in WB, ELISA, ICC, IF (cc), IF (p), IHC (p) and IHC (fro). The antibody is reactive with Human and Rat samples.

Quick Overview for GABPA antibody (AA 51-150) (ABIN1714176)

Target: GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kDa (GABPA))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GABPA Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GABPA/NRF2A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GABPA antibody (ABIN1714176)

Chang, Wu, Xu, Liao, Wu, Cheng: "Scopoletin Protects against Methylglyoxal-Induced Hyperglycemia and Insulin Resistance Mediated by Suppression of Advanced Glycation Endproducts (AGEs) Generation and Anti-Glycation." in: Molecules (Basel, Switzerland), Vol. 20, Issue 2, pp. 2786-801, (2015) (PubMed).

Target Details for GABPA

Target: GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kDa (GABPA))

Alternative Name: GABPA/NRF2A

Background:

Synonyms: E4TF 1A, E4TF1 60, E4TF1A, GA binding protein alpha chain, GA binding protein transcription factor alpha subunit 60 kDa, GA binding protein transcription factor alpha subunit, GA-binding protein alpha chain, GABP A, GABP alpha subunit, GABP alpha subunit, GABP subunit alpha, Gabpa, GABPA_HUMAN, Human nuclear respiratory factor 2 subunit alpha, NFT 2, NFT2, NRF 2, NRF 2A, NRF2, NRF2A, Nuclear respiratory factor 2 alpha subunit, Nuclear respiratory factor 2 subunit alpha, Nuclear respiratory factor 2 subunit alpha, RCH04A07, Transcription factor E4TF1 60, Transcription factor E4TF1 60, Transcription factor E4TF1-60.

Background: The transcription factor GA-binding protein (GABP) is composed of two subunits, the Ets-related GABP-alpha and a GABP-alpha-associated subunit, GABP beta. GABP alpha binds to a specific DNA sequence and GABP beta exists as b1 and b2 splice variants that differ in their C-termini. In primary neuronal cultures, GABP beta is expressed in both the cytoplasm and the nucleus, whereas GABP alpha is expressed mainly in the nucleus. GABP is constitutively expressed as either a GABP alpha beta heterodimer or a GABP alpha b heterotetramer, both of which can modify GABP-dependent transcription in vitro and in vivo. The GABP alpha beta tetrameric complex performs many different functions, such as stimulating transcription of the adenovirus E4 gene, differentially activating BRCA1 expression in human breast cell lines, potentiating Tat-mediated activation of long terminal repeat promoter transcription and viral replication in certain cell types, acting as a coordinator of mitochrondrial and nuclear transcription for cytochrome oxidase in neurons and assisting in the regulation of rpL32 gene transcription.

Gene ID: 2551

Pathways: Myometrial Relaxation and Contraction