RASA1 antibody (pTyr460)

Catalog No. ABIN1714255

This Rabbit Polyclonal antibody specifically detects RASA1 in IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for RASA1 antibody (pTyr460) (ABIN1714255)

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASA1 antibody is un-conjugated

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RASA1 Antibody

Binding Specificity: pTyr460

Purpose: phospho-RASA1 (Tyr460) Polyclonal Antibody

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human GAP around the phosphorylation site of Tyr460

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for RASA1

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Alternative Name: RASA1

Background:

Synonyms: GAP phospho Y460, p-GAP phospho Y460, Ras GAP, CM AVM, CMAVM, DKFZp434N071, GAP, GTPase activating protein, GTPase-activating protein, OTTHUMP00000222390, OTTHUMP00000222391, OTTHUMP00000222392, OTTHUMP00000222393, p120GAP, p120RASGAP, PKWS, Ras GTPase-activating protein 1, RAS p21 protein activator GTPase activating protein 1, Ras p21 protein activator, RASA, RASA1, RASA1_HUMAN, RasGAP, Triphosphatase activating protein.

Background: The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode ubiquitously expressed proteins (1,2). p21Ras can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (3). Oncogenic p21Ras proteins are trapped in the excited signal-emitting state because the mechanism normally employed to delimit their excitation period, hydrolysis of their bound GTP to GDP, is impaired as a result of specific mutations (3). Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold (4,5). The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity (6,7), and proteins that stimulate the GTPase activity of three other low molecular weight GTPases, including Rho, Rab 3A and Rap 1, have also been described (8,9).

Gene ID: 5921

Pathways: Regulation of Actin Filament Polymerization, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals