Actin antibody (pTyr53)

Catalog No. ABIN1714326

This anti-Actin antibody is a Rabbit Polyclonal antibody detecting Actin in WB, ELISA, IHC (p), IF (cc), IF (p), FACS, ICC and IHC (fro). Suitable for Human, Rat and Mouse.

Quick Overview for Actin antibody (pTyr53) (ABIN1714326)

Target: Actin

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Actin Antibody

Binding Specificity: pTyr53

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human beta Actin around the phosphorylation site of Tyr53

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Actin

Target: Actin

Background:

Synonyms: beta Actin phospho Y53, p-beta Actin phospho Y53, A26C1A, A26C1B, ACTB, ACTB_HUMAN, Actin cytoplasmic 1, Actx, beta cytoskeletal actin, Beta-actin, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1.

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene ID: 60