ALKBH1 antibody (AA 1-100)

Catalog No. ABIN1714649

The Rabbit Polyclonal anti-ALKBH1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) and ICC. It is suitable to detect ALKBH1 in samples from Mouse and Rat.

Quick Overview for ALKBH1 antibody (AA 1-100) (ABIN1714649)

Target: ALKBH1 (AlkB, Alkylation Repair Homolog 1 (ALKBH1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-ALKBH1 Antibody

Binding Specificity: AA 1-100

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ABH1/ALKB

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ALKBH1

Target: ALKBH1 (AlkB, Alkylation Repair Homolog 1 (ALKBH1))

Alternative Name: ABH1/ALKB

Background:

Synonyms: ABH, ABH1, alkB, AlkB, alkylation repair homolog 1 E. coli, ALKB1_HUMAN, ALKBH, ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alkylation repair homolog 1, Alkylation repair, alkB homolog, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, hABH.

Background: ALKB protects DNA and RNA against damage from methylating compounds from the environment by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzymes act by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate, and molecular oxygen as the oxidizing agent. Deficiencies in DNA and RNA repair in mammals are associated with cancer, neurological disease and developmental defects. ALKB plays a role in resistance to anti-cancer drugs which attempt to damage tumor DNA. Escherichia coli ALKB protein belongs to the superfamily of 2-oxoglutarate- and iron(II)-dependent oxygenases.

Gene ID: 8846

Pathways: DNA Damage Repair