WDFY1 antibody (AA 21-120)

Catalog No. ABIN1714756

This Rabbit Polyclonal antibody specifically detects WDFY1 in ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) and ICC. It exhibits reactivity toward Rat.

Quick Overview for WDFY1 antibody (AA 21-120) (ABIN1714756)

Target: WDFY1 (WD Repeat and FYVE Domain Containing 1 (WDFY1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDFY1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-WDFY1 Antibody

Binding Specificity: AA 21-120

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human WDFY1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for WDFY1

Target: WDFY1 (WD Repeat and FYVE Domain Containing 1 (WDFY1))

Alternative Name: WDFY1 + WDFY2

Background:

Synonyms: FENS 1, FENS-1, FENS1, KIAA1435, Phosphoinositide binding protein 1, Phosphoinositide binding protein SR1, Phosphoinositide-binding protein 1, WD repeat and FYVE domain containing 1, WD repeat and FYVE domain-containing protein 1, WD40 and FYVE domain containing protein 1, WD40- and FYVE domain-containing protein 1, WDF1, WDFY1, WDFY1_HUMAN, ZFYVE17, Zinc finger FYVE domain containing protein 17, Zinc finger FYVE domain-containing protein 17, WDFY2, WD repeat and FYVE domain-containing protein 2, Propeller-FYVE protein, WD40- and FYVE domain-containing protein 2, Zinc finger FYVE domain-containing protein 22, WDF2, ZFYVE22

Background: WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. WDFY1 positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. WDFY1 Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-beta and inflammatory cytokines. WDFY2 acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2. WDFY2 positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1

Gene ID: 57590, 115825

UniProt: Q8IWB7, Q96P53