SCUBE3 antibody (AA 451-550)

Catalog No. ABIN1714868

The Rabbit Polyclonal anti-SCUBE3 antibody has been validated for ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It is suitable to detect SCUBE3 in samples from Human.

Quick Overview for SCUBE3 antibody (AA 451-550) (ABIN1714868)

Target: SCUBE3 (Signal Peptide, CUB Domain, EGF-Like 3 (SCUBE3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCUBE3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SCUBE3 Antibody

Binding Specificity: AA 451-550

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SCUBE3

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SCUBE3

Target: SCUBE3 (Signal Peptide, CUB Domain, EGF-Like 3 (SCUBE3))

Alternative Name: SCUBE3

Background:

Synonyms: CEGF3, CUB and EGF containing protein 3, CUB and EGF-like domain-containing protein 3, CUB domain and EGF like repeat containing 3, DKFZp686B09105, DKFZp686B1223, DKFZp686D20108, FLJ34743, OTTHUMP00000016250, SCUB3_HUMAN, SCUBE 3, SCUBE3, Signal peptide, Signal peptide CUB and EGF like domain containing protein 3, Signal peptide CUB domain EGF like 3.

Background: SCUBE3 is a novel secreted 993 amino acid cell-surface osteoblast protein that plays an important role in bone cell biology. While highly expressed in osteoblasts, SCUBE3 expression is unobservable or very low in non-bone tissues. SCUBE3 forms homo-oligomers and hetero-oligomers with SCUBE1, and may undergo C-terminal proteolytic cleavage or become N-glycosylated following translation. Two SCUBE3 isoforms exist as a result of alternative splicing events. SCUBE3 contains one CUB domain and nine EGF-like domains, and is encoded by a gene which maps to human chromosome 6p21.31, a region associated with a rare form of metabolic bone disease known as Paget's disease. Chromosome 6 contains 170 million base pairs and comprises nearly 6 % of the human genome.

Gene ID: 222663