Vimentin antibody (AA 371-466)

Catalog No. ABIN1714892

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p) and IF (cc). It is suitable to detect Vimentin in samples from Human, Mouse, Rat and Rabbit. There are 2+ publications available.

Quick Overview for Vimentin antibody (AA 371-466) (ABIN1714892)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Vimentin Antibody

Binding Specificity: AA 371-466

Specificity: KO-Validated

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Vimentin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Vimentin antibody (ABIN1714892)

Yang, Hui, Wang, Yang, Jiang: "Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/?-catenin pathway in laryngeal cancer Hep-2 cells." in: Tumour biology, Vol. 35, Issue 8, pp. 7965-73, (2014) (PubMed).

He, Kou, Luo, Yang, Liu, Wang, Song, Cao, Zeng, Gan, Zhou: "Enhanced M1/M2 Macrophage Ratio Promotes Orthodontic Root Resorption." in: Journal of dental research, (2014) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

Synonyms: VIM, FLJ36605, OTTHUMP00000019224, VIM, VIME_HUMAN, Vimentin.

Background: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Pathways: Caspase Cascade in Apoptosis