ABI1 antibody

Catalog No. ABIN1720813

This Rabbit Polyclonal antibody specifically detects ABI1 in WB and IP. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for ABI1 antibody (ABIN1720813)

Target: ABI1 (Abl-Interactor 1 (ABI1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ABI1 Antibody

Purpose: Anti-ABI1 | Abscisic acid insensitive 1

Specificity: TAIR: At4g26080

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Sorghum bicolor

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide

Application Details

Application Notes: 5 μg (IP for a 200 µL of a cell extract), 3 μg (WB)

Comment:

Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for ABI1

Target: ABI1 (Abl-Interactor 1 (ABI1))

Alternative Name: ABI1

Background:

Background: ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene. Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.

Additional information: It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf. This protein could not be detected using this antibody in plants grown under optimal (non stressed) conditions, The antibody detects both, recombinant and endogenous ABI1 proteins.

Molecular Weight: 47.5 kDa

Gene ID: 828714

UniProt: P49597

Pathways: RTK Signaling, Response to Water Deprivation, ER-Nucleus Signaling