CpxA (N-Term) antibody
Quick Overview for CpxA (N-Term) antibody (ABIN1720869)
Target
Reactivity
Host
Clonality
Application
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Binding Specificity
- N-Term
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Purpose
- Anti-CpxA | Conjugative plasmid expression
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Cross-Reactivity (Details)
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Yersinia pseudotuberculosis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Purification
- Serum
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Immunogen
- Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag
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Application Notes
- 1 : 2000 (WB)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Serum
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- CpxA
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Background
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Background: CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.
Additional information: The strains were grown in LB broth until late stationary phase (absorbance at 600nm of ~1,5), To avoid interference from cross-reacting bands, aim to maximize protein separation with the region of 35 to 55 kDa, CpxA could be routinely detected from a 20 μL of sample that was derived from 1 mL of pelleted bacteria resuspended in 200 μL of 1x loading buffer, Re-use of diluted working strength antibody is not recommended
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Molecular Weight
- 51.8 | 52-53 kDa
Target
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