AGO2 antibody

Catalog No. ABIN1720882

This Rabbit Polyclonal antibody specifically detects AGO2 in WB and IP. It exhibits reactivity toward Arabidopsis thaliana.

Quick Overview for AGO2 antibody (ABIN1720882)

Target: AGO2 (Argonaute 2 (AGO2))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-AGO2 Antibody

Purpose: Anti-AGO2 | Argonaute 2

Specificity: TAIR: At1g48410

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Chlamydomonas reinhardtii, Medicago truncatula, Nicotiana benthamiana, Populus sp., Solanum lycopersicum, Zea mays

Predicted Reactivity: Capsella rubella,Solanum tuberosum

Purification: Immunogen affinity purified

Immunogen: KLH-conjugated synthetic peptide

Application Details

Application Notes: 1 : 250-1 : 500 (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified IgG in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: AGO2

Background: AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). AGO2 is probably involved in antiviral RNA silencing.

Molecular Weight: 113 kDa

Gene ID: 840016

UniProt: Q9SHF3

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization