E2F1 antibody (AA 162-476)

Catalog No. ABIN1721511

This anti-E2F1 antibody is a Mouse Monoclonal antibody detecting E2F1 in WB, IHC, IP, IF and ICC. Suitable for Human.

Quick Overview for E2F1 antibody (AA 162-476) (ABIN1721511)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 18E10

Product Details anti-E2F1 Antibody

Binding Specificity: AA 162-476

Specificity: Reacts with human E2F-1 (aa 244-264).

Purification: Purified (protein G)

Immunogen: GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.

Isotype: IgG1 kappa

Application Details

Application Notes: Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC(1 - 5 μg/mL) IP(1 - 5 μg/mL) IHC(1 - 5 μg/mL) WB(1 - 5 μg/mL)

Positive control: E2F-1 transfected Soas2 cells and recombinant fusion protein

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.4.

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: -80 °C

Storage Comment: This antibody is stable for at least one year at -70°C. Avoid multiple freeze-thaw cycles.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F-1

Background: Transcription factor E2F1 PBR3 Retinoblastoma-associated protein 1, RBAP-1 Retinoblastoma-binding protein 3, RBBP-3 pRB-binding protein E2F-1 Gene name : E2F1

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy