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GFP antibody (Green Fluorescent Protein) Primary Antibody

GFP Reactivity: Aequorea victoria FACS, IHC, ELISA, WB Host: Mouse Monoclonal 4B10B2 unconjugated
Pubmed (3)
Catalog No. ABIN1724673
$359.70
Plus shipping costs $45.00
100 μL
local_shipping Shipping to: United States
Delivery in 3 to 4 Business Days
  • Target
    GFP
    Reactivity
    • 187
    • 18
    • 15
    • 15
    • 6
    • 5
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Aequorea victoria
    Host
    • 75
    • 73
    • 32
    • 15
    • 12
    • 6
    • 3
    • 1
    Mouse
    Clonality
    • 124
    • 89
    Monoclonal
    Conjugate
    • 121
    • 11
    • 10
    • 10
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GFP antibody is un-conjugated
    Application
    • 157
    • 96
    • 46
    • 46
    • 38
    • 36
    • 30
    • 22
    • 16
    • 15
    • 12
    • 12
    • 11
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Purification
    Purified
    Immunogen
    Purified recombinant fragment of GFP expressed in E. Coli.
    Clone
    4B10B2
    Isotype
    IgG2a
  • Application Notes
    ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS containing 0.03 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    4°C, -20°C for long term storage
  • Dittman, Kaplan: "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11399-404, 2006 (PubMed).

    Cavalieri, Bernardo, Spinelli: "Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells." in: Gene expression patterns : GEP, Vol. 7, Issue 1-2, pp. 124-30, 2006 (PubMed).

    Arasada, Son, Ramalingam, Eichinger, Schleicher, Rohlfs: "Characterization of the Ste20-like kinase Krs1 of Dictyostelium discoideum." in: European journal of cell biology, Vol. 85, Issue 9-10, pp. 1059-68, 2006 (PubMed).

  • Target
    GFP
    Alternative Name
    GFP
    Synonyms
    green fluorescent protein, gfp
    Target Type
    Viral Protein
    Background
    GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
    Molecular Weight
    27 kDa
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