RCHY1 antibody

Catalog No. ABIN1724723

There are 2+ publications for this product available. The Mouse Monoclonal anti-RCHY1 antibody is suitable to detect RCHY1 in samples from Human and Rat. It has been validated for WB, ELISA, IHC, ICC and FACS.

Quick Overview for RCHY1 antibody (ABIN1724723)

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RCHY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1H10

Product Details anti-RCHY1 Antibody

Purpose: Pirh2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human Pirh2 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-RCHY1 antibody (ABIN1724723)

Duan, Gao, Wu, Zhang, Otterson, Villalona-Calero: "Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells." in: Experimental cell research, Vol. 312, Issue 17, pp. 3370-8, (2006) (PubMed).

Duan, Gao, Druhan, Zhu, Morrison, Otterson, Villalona-Calero: "Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer." in: Journal of the National Cancer Institute, Vol. 96, Issue 22, pp. 1718-21, (2004) (PubMed).

Target Details for RCHY1

Target: RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))

Alternative Name: Pirh2

Background: Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.

Molecular Weight: 30 kDa, 60 kDa (homodimer)

Gene ID: 25898

UniProt: Q96PM5