GLUT4 antibody (AA 224-353)

Catalog No. ABIN1724743

This Mouse Monoclonal antibody specifically detects GLUT4 in WB, IHC, ELISA, ICC and FACS. It exhibits reactivity toward Human and Mouse.It has been mentioned in 1 publication

Quick Overview for GLUT4 antibody (AA 224-353) (ABIN1724743)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3G10A3

Product Details anti-GLUT4 Antibody

Binding Specificity: AA 224-353

Purpose: SLC2A4 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human SLC2A4 (AA: 224-353 ) expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-GLUT4 antibody (ABIN1724743)

Sano, Peck, Kettenbach, Gerber, Lienhard: "Insulin-stimulated GLUT4 protein translocation in adipocytes requires the Rab10 guanine nucleotide exchange factor Dennd4C." in: The Journal of biological chemistry, Vol. 286, Issue 19, pp. 16541-5, (2011) (PubMed).

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: SLC2A4

Background: This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

Molecular Weight: 54.8 kDa

Gene ID: 6517

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect