EGFR antibody (AA 693-893)

Catalog No. ABIN1724890

This Mouse Monoclonal antibody specifically detects EGFR in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for EGFR antibody (AA 693-893) (ABIN1724890)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 7A6F12

Product Details anti-EGFR Antibody

Binding Specificity: AA 693-893

Purpose: EGFR Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-EGFR antibody (ABIN1724890)

Derer, Bauer, Lohse, Scheel, Berger, Kellner, Peipp, Valerius: "Impact of epidermal growth factor receptor (EGFR) cell surface expression levels on effector mechanisms of EGFR antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 11, pp. 5230-9, (2012) (PubMed).

Minder, Bayha, Becker-Pauly, Sterchi: "Meprin? transactivates the epidermal growth factor receptor (EGFR) via ligand shedding, thereby enhancing colorectal cancer cell proliferation and migration." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35201-11, (2012) (PubMed).

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Molecular Weight: 175 kDa

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins