PRKAG1 antibody (AA 230-331)

Catalog No. ABIN1724935

This Mouse Monoclonal antibody specifically detects PRKAG1 in ELISA and IHC. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for PRKAG1 antibody (AA 230-331) (ABIN1724935)

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKAG1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Clone: 4A1G9

Product Details anti-PRKAG1 Antibody

Binding Specificity: AA 230-331

Purpose: PRKAG1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human PRKAG1 (AA: 230-331) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-PRKAG1 antibody (ABIN1724935)

Zaha, Young: "AMP-activated protein kinase regulation and biological actions in the heart." in: Circulation research, Vol. 111, Issue 6, pp. 800-14, (2012) (PubMed).

Oliveira, Zhang, Solis, Isackson, Bellahcene, Yavari, Pinter, Davies, Ge, Ashrafian, Walker, Carling, Watkins, Casadei, Redwood: "AMP-activated protein kinase phosphorylates cardiac troponin I and alters contractility of murine ventricular myocytes." in: Circulation research, Vol. 110, Issue 9, pp. 1192-201, (2012) (PubMed).

Target Details for PRKAG1

Target: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Alternative Name: PRKAG1

Background: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molecular Weight: 37.6 kDa

Gene ID: 5571

UniProt: P54619

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process, Warburg Effect