Amyloid Fibrils antibody

Catalog No. ABIN1731752

The Rabbit Polyclonal anti- antibody has been validated for WB, DB, ELISA and IHC. It is suitable to detect in samples from Human, Mouse and Rat.

Quick Overview for Amyloid Fibrils antibody (ABIN1731752)

Target: Amyloid Fibrils

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Dot Blot (DB), ELISA, Immunohistochemistry (IHC)

Product Details

Specificity: Reacts withepitopes common to many human amyloid fibrils and fibrillar oligomers but not prefibrillar oligomers or natively folded proteins. Predicted to recognize mouse and rat based on sequence homology.

Purification: Purified

Immunogen: Fibrils prepared from human Abeta42 peptide

Isotype: IgG

Application Details

Application Notes: Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
WB1 -2 μg/mL DB0.5 - 1 μg/mL IHC1 -5 μg/mL ELISA1 - 10 μg/mL with amyloid fibril-containing samples on the solid phase.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS pH 7.0, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute in PBS or medium which is identical to that used in the assay system.

Storage: -20 °C

Storage Comment: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Target Details

Target: Amyloid Fibrils

Background: Amyloid monomeric proteins can oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibers.