ATG4B antibody

Catalog No. ABIN1732017

This anti-ATG4B antibody is a Rabbit Polyclonal antibody detecting ATG4B in WB and IHC (p). Suitable for Human, Zebrafish (Danio rerio), Chicken, Xenopus laevis and Mammalian.

Quick Overview for ATG4B antibody (ABIN1732017)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human, Zebrafish (Danio rerio), Chicken, Xenopus laevis, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG4B Antibody

Specificity: Reacts with human ATG4B

Cross-Reactivity: Mouse (Murine), Zebrafish, Chicken, Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with chicken, xenopus, mouse, pufferfish, rat and zebrafish protein.

Purification: Purified (protein G)

Immunogen: Synthetic peptide from human ATG4B.

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, 0.2 % gelatin, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy.

Gene ID: 23192

Pathways: Autophagy