Neuroligin 1 antibody (AA 718-843) (APC)

Catalog No. ABIN1741315

This Mouse Monoclonal antibody specifically detects Neuroligin 1 in WB, IHC, ICC and IF. It exhibits reactivity toward Rat.

Quick Overview for Neuroligin 1 antibody (AA 718-843) (APC) (ABIN1741315)

Target: Neuroligin 1 (NLGN1)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neuroligin 1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S97A-31

Product Details anti-Neuroligin 1 Antibody (APC)

Binding Specificity: AA 718-843

Specificity: Detects ~120 kDa. Does not cross-react with other Neuroligins.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3.

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

2 μg/ml of ABIN1741315 was sufficient for detection of Neuroligin-1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Neuroligin 1

Target: Neuroligin 1 (NLGN1)

Alternative Name: Neuroligin 1

Background: Neuroligin-1 is a neuronal cell surface protein belonging to the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses for their normal, functional development, and is necessary to the regulation of synaptic plasticity and the development of long-term memory within the adult amygdala in mammals. It is believed to participate in cell-cell-interaction through the assembly of intracellular junction by the binding of beta-neurexins, and may also be a factor in the maintenance and assembly of synaptic junctions. It is also thought to have involvement in excitatory synaptic specification. Within brain tissue, Neuroligin-1 is primarily observed in neurons and spinal cord.

Gene ID: 116647

UniProt: Q62765

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis