HSF1 antibody (AA 425-439) (Atto 390)

Catalog No. ABIN1741558

This Rat Monoclonal antibody specifically detects HSF1 in WB, ELISA, IHC, IP, IF, GS and ICC. It exhibits reactivity toward Mouse.

Quick Overview for HSF1 antibody (AA 425-439) (Atto 390) (ABIN1741558)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This HSF1 antibody is conjugated to Atto 390

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)

Clone: 4B4

Product Details anti-HSF1 Antibody (Atto 390)

Binding Specificity: AA 425-439

Specificity: Detects ~85 kDa (unstressed cell lysates) and ~95 kDa (heat shocked cell lysates).

Cross-Reactivity: Cow, Guinea Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat

Purification: Protein G Purified

Immunogen: Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741558 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Gene ID: 15499

NCBI Accession: NP_032322

UniProt: P38532