POLR2A/RPB1 antibody

Catalog No. ABIN1742178

The Rabbit Polyclonal anti-POLR2A/RPB1 antibody (ABIN1742178) specifically detects POLR2A/RPB1 in IP and ChIP. The antibody is reactive with Arabidopsis thaliana samples.

Quick Overview for POLR2A/RPB1 antibody (ABIN1742178)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Product Details anti-POLR2A/RPB1 Antibody

Purpose: Anti-RPB1 | RNA polymerase II subunit B1

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Physcomitrium patens

Predicted Reactivity: Amygdalus persica,Asparagus officinalis,Brassica rapa,Capsella rubella,Glycine max,Hordeum vulgare,Solanum lycopersicum,Oryza glaberrima,Panicum italicum,Persea americana,Pisum sativum,Ricinus communis,Secale cereale,Solanum tuberosum,Sorghum vulgare,Triticum aestivum,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences

Application Details

Application Notes: 1,2 or 5 μg (ChIP, IP)

Comment:

Purified IgG was lyophilized from PBS pH 7.4 + 0.02 % sodium azide. Therefore only distilled water is required for its re-constitution.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: RNA polymerase II subunit B1

Background:

Background: DNA-directed RNA polymerase II subunit 1 (EC=2.7.7.6) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This enzyme is the central component of the basal RNA polymerase II transcription machinery. Synonymes:DNA-directed RNA polymerase III largest subunit,DNA-directed RNA polymerase II subunit RPB1.

Additional information: Purified IgG was lyophilized from PBS pH 7,4 + 0,02 % sodium azide, Therefore only distilled water is required for its re-constitution

Molecular Weight: 204 kDa

Gene ID: 829734

UniProt: P18616

Pathways: Regulatory RNA Pathways