SYT1 antibody (AA 1-8)

Catalog No. ABIN1742201

The Guinea Pig Polyclonal anti-SYT1 antibody has been validated for WB, IHC and ICC. It is suitable to detect SYT1 in samples from Rat. There is 1 publication available.

Quick Overview for SYT1 antibody (AA 1-8) (ABIN1742201)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-SYT1 Antibody

Binding Specificity: AA 1-8

Purpose: anti-Synaptotagmin1

Specificity: Specific for synaptotagmin 1

Cross-Reactivity (Details): Reacts with: rat (P21707). Other species not tested yet.

Purification: affinity purified

Immunogen: Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)

Application Details

Application Notes: WB:1 : 2000 , IP:not tested yet ICC:1 : 500, IHC:1 : 500, IHC-P:not tested yet

Comment:

This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin was added for stabilization.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin was added for stabilization. Do not freeze!

References for anti-SYT1 antibody (ABIN1742201)

Shinoda, Ahmed, Ramachandran, Bharat, Brockelt, Altas, Dean: "BDNF enhances spontaneous and activity-dependent neurotransmitter release at excitatory terminals but not at inhibitory terminals in hippocampal neurons." in: Frontiers in synaptic neuroscience, Vol. 6, pp. 27, (2014) (PubMed).

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin1

Background: P65, Syt1, SynaptotagminI, SytI

UniProt: P46096

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport