SNAP25 antibody (AA 192-206)

Catalog No. ABIN1742235

This anti-SNAP25 antibody is a Rabbit Polyclonal antibody detecting SNAP25 in WB, ELISA, IHC, IP and ICC. Suitable for Human, Rat, Mouse, Chicken, Zebrafish (Danio rerio), Hamster and Goldfish. This Primary Antibody has been cited in 9+ publications.

Quick Overview for SNAP25 antibody (AA 192-206) (ABIN1742235)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human, Rat, Mouse, Chicken, Zebrafish (Danio rerio), Hamster, Goldfish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-SNAP25 Antibody

Binding Specificity: AA 192-206

Specificity: Specific for SNAP 25.

Purification: antiserum

Immunogen: Synthetic peptide IDEANQRATKMLGSG (aa 192-206 in human SNAP 25) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 10000 (AP staining)
ICC: 1 : 100

Comment:

Recognizes the Botulinum neurotoxin A cleavage product with reduced affinity. Does not detect the neurotoxin E cleavage product. Recognizes splice variants SNAP 25A and B. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SNAP25 antibody (ABIN1742235)

Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).

Fossati, Morini, Corradini, Antonucci, Trepte, Edry, Sharma, Papale, Pozzi, Defilippi, Meier, Brambilla, Turco, Rosenblum, Wanker, Ziv, Menna, Matteoli: "Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis." in: Cell death and differentiation, Vol. 22, Issue 9, pp. 1425-36, (2015) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Young, Franciosi, Spreeuw, Deng, Sanders, Tam, Huang, Singaraja, Zhang, Bissada, Kay, Hayden: "Low levels of human HIP14 are sufficient to rescue neuropathological, behavioural, and enzymatic defects due to loss of murine HIP14 in Hip14-/- mice." in: PLoS ONE, Vol. 7, Issue 5, pp. e36315, (2012) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Camoletto, Vara, Morando, Connell, Marletto, Giustetto, Sassoè-Pognetto, Van Veldhoven, Ledesma: "Synaptic vesicle docking: sphingosine regulates syntaxin1 interaction with Munc18." in: PLoS ONE, Vol. 4, Issue 4, pp. e5310, (2009) (PubMed).

Johansson, Ericsson, Janson, Beraki, Stanić, Mandic, Wikström, Hökfelt, Ogren, Rozell, Berggren, Bark: "An ancient duplication of exon 5 in the Snap25 gene is required for complex neuronal development/function." in: PLoS genetics, Vol. 4, Issue 11, pp. e1000278, (2008) (PubMed).

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP 25

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport