SV2B antibody (AA 2-17)

Catalog No. ABIN1742257

This Mouse Monoclonal antibody specifically detects SV2B in WB, IHC, ICC and IP. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for SV2B antibody (AA 2-17) (ABIN1742257)

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SV2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: 246E8

Product Details anti-SV2B Antibody

Binding Specificity: AA 2-17

Specificity: Specific for SV2 B.

Purification: purified IgG

Immunogen: Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC: 1 : 200
IHC-P: 1 : 500

Comment:

WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide (= 0.02 µg)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SV2B antibody (ABIN1742257)

Aroeira, Vaz, Sebastião, Valente: "BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2." in: Neurochemistry international, Vol. 99, pp. 94-102, (2016) (PubMed).

Dagar, Nagar, Goel, Cherukuri, Dhingra: "Loss of photoreceptors results in upregulation of synaptic proteins in bipolar cells and amacrine cells." in: PLoS ONE, Vol. 9, Issue 3, pp. e90250, (2014) (PubMed).

Target Details for SV2B

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Alternative Name: SV2 B