SV2B antibody (AA 2-17)

Catalog No. ABIN1742258

The Rabbit Polyclonal anti-SV2B antibody has been validated for WB, IHC, IHC (p), ICC and IP. It is suitable to detect SV2B in samples from Human, Mouse, Rat, Hamster and Chicken. There are 17+ publications available.

Quick Overview for SV2B antibody (AA 2-17) (ABIN1742258)

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Reactivity: Human, Mouse, Rat, Hamster, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SV2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-SV2B Antibody

Binding Specificity: AA 2-17

Purpose: Rabbit anti-SV2 B antiserum

Specificity: Specific for SV2 B.

Cross-Reactivity (Details): Reacts with: human (Q7L1I2), rat (Q63564), mouse (Q8BG39), hamster, chicken. Other species not tested yet.

Purification: antiserum

Immunogen: Synthetic peptide corresponding to AA 2 to 17 from rat SV2B (UniProt Id: Q63564)

Application Details

Application Notes: WB:1 : 1000

Comment:

WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Do not freeze!

References for anti-SV2B antibody (ABIN1742258)

Fukusumi, Wakamatsu, Takashima, Hasegawa, Miyauchi, Tomita, Kawachi: "SV2B is essential for the integrity of the glomerular filtration barrier." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 5, pp. 534-45, (2015) (PubMed).

Crèvecoeur, Kaminski, Rogister, Foerch, Vandenplas, Neveux, Mazzuferi, Kroonen, Poulet, Martin, Sadzot, Rikir, Klitgaard, Moonen, Deprez: "Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis." in: Neuropathology and applied neurobiology, Vol. 40, Issue 2, pp. 191-204, (2014) (PubMed).

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Mahrhold, Strotmeier, Garcia-Rodriguez, Lou, Marks, Rummel, Binz: "Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E." in: The Biochemical journal, Vol. 453, Issue 1, pp. 37-47, (2013) (PubMed).

Crèvecœur, Foerch, Doupagne, Thielen, Vandenplas, Moonen, Deprez, Rogister: "Expression of SV2 isoforms during rodent brain development." in: BMC neuroscience, Vol. 14, pp. 87, (2013) (PubMed).

Couesnon, Molgó, Connan, Popoff: "Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine." in: PLoS pathogens, Vol. 8, Issue 3, pp. e1002583, (2012) (PubMed).

Lazarevic, Schöne, Heine, Gundelfinger, Fejtova: "Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 28, pp. 10189-200, (2011) (PubMed).

Boido, Farisello, Cesca, Ferrea, Valtorta, Benfenati, Baldelli: "Cortico-hippocampal hyperexcitability in synapsin I/II/III knockout mice: age-dependency and response to the antiepileptic drug levetiracetam." in: Neuroscience, Vol. 171, Issue 1, pp. 268-83, (2010) (PubMed).

Yeh, Dong, Yao, Tepp, Lin, Johnson, Chapman: "SV2 mediates entry of tetanus neurotoxin into central neurons." in: PLoS pathogens, Vol. 6, Issue 11, pp. e1001207, (2010) (PubMed).

Morgans, Kensel-Hammes, Hurley, Burton, Idzerda, McKnight, Bajjalieh: "Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina." in: PLoS ONE, Vol. 4, Issue 4, pp. e5230, (2009) (PubMed).

Couesnon, Pereira, Popoff: "Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers." in: Cellular microbiology, Vol. 10, Issue 2, pp. 375-87, (2008) (PubMed).

Verderio, Grumelli, Raiteri, Coco, Paluzzi, Caccin, Rossetto, Bonanno, Montecucco, Matteoli: "Traffic of botulinum toxins A and E in excitatory and inhibitory neurons." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 2, pp. 142-53, (2007) (PubMed).

Miyauchi, Saito, Karasawa, Harita, Suzuki, Koike, Han, Shimizu, Kawachi: "Synaptic vesicle protein 2B is expressed in podocyte, and its expression is altered in proteinuric glomeruli." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 10, pp. 2748-59, (2006) (PubMed).

Schivell, Mochida, Kensel-Hammes, Custer, Bajjalieh: "SV2A and SV2C contain a unique synaptotagmin-binding site." in: Molecular and cellular neurosciences, Vol. 29, Issue 1, pp. 56-64, (2005) (PubMed).

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Redecker, Kreutz, Bockmann, Gundelfinger, Boeckers: "Brain synaptic junctional proteins at the acrosome of rat testicular germ cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 51, Issue 6, pp. 809-19, (2003) (PubMed).

Janz, Südhof: "SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family." in: Neuroscience, Vol. 94, Issue 4, pp. 1279-90, (2000) (PubMed).

Target Details for SV2B

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Alternative Name: SV2 B

Background:

Synonyms: synaptic vesicle protein 2B, SV2, SV2-B, synaptic vesicle protein 2

Antigen Information: K.O. SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters

UniProt: Q63564